dhfr
释义
[医][=dihydrofolate reductase]二氢叶酸还原酶;
例句:
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After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~ - ) cells.
经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.
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A mammalian expression vector ( pSV _ 2 , - EP ) was reconstructed by inserting a oligonucleolide fragment into pSV _ 2 dhfr plasmid.
对pSV_2dhfr质粒进行改造,切除dhfr基因,加入一个人工合成的寡聚核苷酸片段,构建成哺乳动物细胞表达载体 ( pSV_2-EP ).
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SDS - PAGE showed that the fused protein of interest has been specifically expressed as 61 kD.
所得菌体总蛋白经SDS -PAGE 分析,结果表明,在预期的61kD处 得到一条特异带,为AK( 1-3)cDNA与 载体固有基因DHFR-6хHis的重组蛋白表达产物.